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In brief, the FHbp gene was replaced by an erythromycin resistance cassette carried on pBSΔgna1870erm (5). ….. 2C]). In experiment 2, a 1:40 dilution of an anti-FHbp serum pool from mice immunized with FHbp ID 4, which matched the FHbp sequence variant of the R1/S1 isolate pair, conferred protection against both …
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However, in those pa- tients for whom either no mutation or only one mutation was found by use of this strategy, all exons were se- quenced. This resulted in a mutational detection rate of. 96%. In all patients with biochemically confirmed SLOS, we found. 1 mutation. For only six patients were we unable to identify a 2d …